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CRISPR Target Assessment

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Features and additional functionalities are constantly added to CRISTA.
If you encounter problems, please let us know.

Predict your sgRNA::DNA pairs scores

CRISTA accounts for the possibility of bulges and incorporates a wide range of features encompassing those that are specific to the genomic content, features that define the thermodynamics of the sgRNA, and features concerning the pairwise similarity between the sgRNA and the genomic target.
The features are computed according to the sgRNA and genomic coordinates or context you specify as input here, below.

Type a 20-nt sgRNA:  

Target input type: Genomic coordinates    Genomic site sequence   

Type the genomic site sequence (29-nt):
3-nt flanking sequences upstream and downstream




Alternatively, you may upload a file with multiple records (according to this template):

Genome assembly: 
230 genome assemblies are available, including vertebrates, plants, yeast, insects and deuterostomes!
Is your reference of choice missing? Please let us know.


Chromosome:             Write a legitimate chromosome number, e.g., 1, 22, X, Y

Strand:                    +    -   

5' start coordinate:  



Alternatively, you may upload a file with multiple records (according to this template):


Please enter your email address
Your email address will be used to update you the moment the results are ready.